Request for Plasmids
 

PKC constructs available for request:

Mutants Tag PKCa PKCb1 PKCb2 PKCg PKCd PKCe PKCh PKCq PKCz PKCi Reference
Wild Type (WT) C-terminal HA pHACE pHACB pHACB pHACE pHACE pHACE pHACE - pHACE pHACE 1, 3
C-terminal EGFP pGFP3 pGFP3 pGFP3 pGFP3 pGFP3 pGFP3 pGFP3 - pGFP3 pGFP3 5
Dominant negative mutant (DN) C-terminal HA pHACE pHACB pHACB pHACE pHACE pHACE pHACE - pHACE pHACE 1, 3
N-terminal PS domain deletion mutant (DNPS)  C-terminal HA pHACE pHACB pHACB pHACE pHACE pHACE pHACE - pHACE pHACE 3
Regulatory domain mutant (REG) N-terminal HA pHAN1 - - - - pHAN1 - - pHAN1 - 2
C-terminal HA - - - - pHACE - - - - - 4
Catalytic domain mutant (CAT) C-terminal HA pHACE pHACB pHACB pHACE pHACE pHACE pHACE - pHACE pHACE 3
N-terminal HA pHANE - - - pHANE pHANE - - pHANE - 1
Kinase dead catalytic domain mutant (CAT-KR) N-terminal HA pHANE - - - pHANE pHANE - - pHANE - 1

 

PKC-specific substrate

GST-MARCKS: pGEX-MARCKS(96-184)

Dominant negative c-Raf mutant: pcDNA3-c-Raf-K375M

 

Notes:

pHACE : mammalian expression vector for a C-terminal HA epitope tag (EcoRI cloning site) (1)

pHACB : mammalian expression vector for a C-terminal HA epitope tag (BamHI cloning site) (1)

pHANE : mammalian expression vector for an N-terminal HA epitope tag (EcoRI cloning site) (1)

pHANB : mammalian expression vector for an N-terminal HA epitope tag (BamHI cloning site) (1)

pHAN1 : mammalian expression vector for an N-terminal HA epitope tag (EcoRI cloning site) (2)

pGFP3  : mammalian expression vector for an C-terminal EGFP tag (EcoRI & BamHI cloning sites) (5)

 

References:

1. Soh, J. W., Lee, E. H., Prywes, R., and Weinstein, I. B. (1999). Novel roles of specific isoforms of protein kinase C in activation of the c-fos serum response element, Mol Cell Biol 19, 1313-24. (PubMed)

2. Soh, J. W., Lee, Y. S., and Weinstein, I. B. (2003). Effects of regulatory domains of specific isoforms of protein kinase C on growth control and apoptosis in MCF-7 breast cancer cells. J Exp Ther Oncol 3, 115-126. (PubMed)

3. Soh, J. W., and Weinstein, I. B. (2003). Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. J Biol Chem 278, 34709-34716. (PubMed)

4. Lee, Y. J., Lee, D. H., Cho, C. K., Bae, S., Jhon, G. J., Lee, S. J., Soh, J. W., and Lee, Y. S. (2005). HSP25 inhibits protein kinase Cdelta-mediated cell death through direct interaction. J Biol Chem 280, 18108-18119. (PubMed)

5. Kazi, J.U., and Soh, J.W. (2007). Isoform-Specific Translocation of PKC Isoforms in NIH3T3 Cells by TPA. Biochem Biophys Res Commun 364, 231-237. (PubMed)

Procedures of plasmid request

1. Send a request letter to Dr. Jae-Won Soh by e-mail.

2. Fill out and send the Material Transfer Agreement Form to Dr. Jae-Won Soh by fax (+82-32-872-6698).