PKC Constructs-expression level

PKC mutants constructed for PKC studies and their Expression level (stability) in COS-7 cells

PKC Constructs	PKC-HA						.	PKC-GFP	PKC-GFP
Vectors	pHACE or pHACB						.	pGFP3	pRFP3
PKC Mutants	WT	KR	DPS	DNPS	CAT	CAT-KR		WT	WT
Mutation (Diagram)	wild type	kinase inactive	pseudosubstrate sequence deletion	N-terminal pseudosubstrate domain deletion	catalytic domain	catalytic domain mutant (kinase inactive)		wild type	wild type
PKCa	++	++	++	++	++	++		+	+
PKCb1	++	++	N/A	+	++	N/A		+	+
PKCb2	++	++	N/A	++	++	N/A		+	+
PKCg	++	++	N/A	++	++	N/A		+	+
PKCd	++	++	+	-	+	+		+	+
PKCe	++	++	+	+	++	++		+	+
PKCh	++	++	N/A	++	++	N/A		+	+
PKCz	++	++	++	++	++	++		+	+
PKCi	++	++	N/A	+	+	N/A		+	+
Data	Structures of the PKC mutants			Coding sequences of the PKC mutants				Fluorescence microscopy	Fluorescence microscopy
	Expression level in COS-7 cells
	Western blot data			Kinase assay data
	Immunofluorescence microscopy			Test digestion protocol
WT: wild type KR: kinase inactive mutant (dominant negative) DPS: pseudosubstrate sequence deletion mutant (constitutively active) DNPS: N-terminal pseudosubstrat domain deletion mutant (constitutively active) CAT: catalytic domain mutant (constitutively active) CAT-KR: catalytic domain mutant (kinase inactive)